Protein activities tend to match with subcellular expressions. Therefore, localization could be done by using fluorescence microscopy by using reporter proteins or dyes such as Alexa. Given the little knowledge in other mitochondrial targeting signals during localization, the proposed intervention would seek to demonstrate how a nucleus-encoded pre-protein could be localized into the intermembrane spaces of the mitochondria through an ATP hydrolysis. This would pave way for further analysis to be done in investigating the specific molecular function as well as its structural and energy-basis for interacting with other membranes.
Mitochondrial hemes do not have an N-terminal targeting information site. The proteins are often essential in the covalent alignment of the c cytochromes. As a result, the import of the heme lyases is possible and easy ( Calvo et al., 2017) . Standard procedures in manipulating the DNA and PCR would be adhered to and Fungal models such as yeast would be used in the proposed process in understanding human health and disease. Targeting signals of the mitochondrial on almost every protein would be identified. The sequence is often highly conserved and hydrophilic in nature. This is due to the presence of both positive and negative chemical charges on the amino acids. As a result, the heme lyases can be easily distinguished from other pre-sequences. When inserted into a cytosolic protein, the targeting sequence directs the membrane that is to be fused into the inter-membrane space that was targeted. The import is among the first topogenic signal that harbors two different types of information in independent energy transport . Translocation is done through an accurate recognition of the mitochondrial outer membrane. Through a series of high energy reactions, the import is easily driven into the inter-membrane space as it was originally intended.
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Mitochondria are considered to be the powerhouse of any body function in living organisms. Translocation of protein into inter-membrane spaces of the mitochondria would have insignificant effects on cell growth. However, the application of excessive amounts of external heat in the facilitation of the ATP hydrolysis would damage the cells. Therefore, the process should be exposed to moderate amounts of an external factor in order not to interfere with the natural mechanism of the cells. Despite this, there would no change in the characteristics of the tissue and organism level. Abnormal translocation would cause human diseases such as cystic fibrosis or hypercholesterolemia among others depending on the type of host tissue the process was being done on ( Herst, Rowe, Carson & Berridge, 2017). On the other hand, NAC in yeast may interact with precursor proteins to cause ribosome docking that would cause protein targeting to the mitochondria surface difficult.
The process of translocation reveals a careful and precise mannerism at which human beings have been created. It exacts that there was some source of superior intelligence that meticulously planted the right form of protein into its respective space to achieve its intended function. As recorded in Psalms 139, we are fearfully and wonderfully created in a unique way that no man can achieve ( Francis, Smith & Corio, 2018). The design in which human beings have been made would ineffectively be altered by man as it demonstrates how humans are vastly different in so many ways. Altering what designed without carefully analyzing the process of creation would likely contribute to sickness or even death. The Uniqueness of the creation of human being provides that science cannot surpass God’s design of the internal structure and functions of the body.
References
Calvo, S. E., Julien, O., Clauser, K. R., Shen, H., Kamer, K. J., Wells, J. A., & Mootha, V. K. (2017). Comparative analysis of mitochondrial N-termini from mouse, human, and yeast. Molecular & Cellular Proteomics , 16 (4), 512-523.
Francis, L. J., Smith, G., & Corio, A. S. (2018). Exploring Psalm 139 through the Jungian lenses of sensing, intuition, feeling and thinking. HTS Teologiese Studies/Theological Studies , 74 (1).
Herst, P. M., Rowe, M. R., Carson, G. M., & Berridge, M. V. (2017). Functional mitochondria in health and disease. Frontiers in endocrinology , 8 , 296.
