Yeast has the highest concentration of glucose. When the S. cerevisiae cells divide rapidly in a glucose medium to process glucose they amalgamate (adenosine triphosphate) ATP through glycolysis. With this, there is ethanol as the by-product (Futcher, 2006). Cells that multiply at a slower rate in one medium shall have a small population of the cells than the medium that multiplies fast (Takeda et al. 2015). Nonetheless, investigations on the Schizosaccharomyces pombe demonstrate a complicated circumstance (Pluskal et al. 2011), indicating that the cells were put in the medium with lower glucose amounts, including 0.004, multiplying faster compared those in the medium with 1 percent glucose. Even though the various Yeast types in low glucose can isolate as fast as cells in the 1 percent medium are unknown hence (Jonhstone et al., 2009). This study aims to establish if yeast cells in a 0.04 percent glucose concentration multiply at the same rate as the ones in the divide just as a 1 percent glucose medium.
Methods and Materials
The o.ml of Yeast with 2 percent glucose was put into a test tube having distilled water. 10ml of the sample was placed into the hemacytometer and allowed to settle for 1 minute. A light microscope was applied in determining the average cell count. The outcome was used to attain the proportion of the percentage of the dividing cells. The cell density was established with the spectrophotometer on an OD₆₀₀ application.
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Results
In the 1 percent concentration, the average proportion of the dividing cells was as 58.595% and 33.759% for Yeast in 0.04% glucose concentration. The results depicted a significant divergence in the percentage of the dividing cells (P-Value = 3E-7)
| Glucose Concentration |
Proportion of Diving Cells |
| 1 | 58.595% |
| 0.04 | 33.759% |
Discussion
The assumption that the yeast cells in the 0.04 percent glucose concentration multiply as fast as those in the 1 percent medium was not proved correct in the research. The outcomes showed a considerable disparity in cell division rates and cell density between the high glucose medium and the low glucose medium. Different results have been reported for the yeast cells maintained in the low and high glucose concentrations medium (Pluskal et al. 2011). Johnston et al. (2007) indicated that glucose concentrations' effect on the cells' proportion density and divisions in the yeast. This study's outcome has proved to be different as we utilized the light microscope in counting the cell division, whereas other studies utilized the microfluid system.
Conclusion
The study investigates glucose concentrations' effect on the cells' proportion density and divisions in the yeast Saccharomyces cerevisiae. The outcome indicated a significant difference in the Yeast's division rate and density kept in the low and high concentrations. One challenge of the research is that through concentration on the single species, future research should be done with various yeast species to establish if the outcome of this research represents all other types of yeasts in the different glucose concentrations. More studies can be done to show how yeast cell size is affected by glucose concentration and also its impact on the population size on those mediums.
References
Futcher B. 2006. Metabolic cycle, cell cycle, and the finishing kick to Start. Genome Biology 7:107.
Johnston, G. C., Pringle, J. R., & Hartwell, L. H. (2007). Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Experimental cell research , 105 (1), 79-98
Johnston, G. C., Ehrhardt, C. W., Lorincz, A., & Carter, B. L. (2009). Regulation of cell size in the yeast Saccharomyces cerevisiae. Journal of bacteriology , 137 (1), 1-5.
Pluskal T, Hayashi T, Saitoh S, Fujisawa A, Yanagida M. 2011 Specific biomarkers for stochastic division patterns and starvation- induced quiescence under limited glucose levels in fission yeast. FEBS Journal 278:1299-1315.
Takeda K, Starzynski C, Mori A, Yanagida M. 2015. The critical glucose concentration for respiration-independent proliferation of fission yeast, Schizosaccharomyces pombe. Mitochondrion 22:91-95.
