Introduction
Aldabbagh et al. 2015 experimented on the effect of different types of disinfectants and antiseptics on four distinct strains of bacteria. Escherichia Coli was one of the bacteria, and blech was one of the agents used. From the investigation, the conclusion was that bleach was active in dealing with the distinct bacteria under investigation. The remaining bacteria in the study are Staphylococcus aureus and Pseudomonas aeruginosa . One of the aims of the investigation of to realize the implications that is available on the bacteria. Another objective is to determine and know the level of resistance that the bacteria has on the agent through the dilution process. There are several learning outcomes for the experiment. One is to be able to recognize and know the strains that the bleach/Clorox has on the efficacy level. Another learning outcome is to be able to know the level of sensitivity that the bacteria has on the agent. The last outcome will be to know the antibiotic resistance level of the bacteria. According to Han et al. 2015, hospitals and other healthcare facilities use disinfectants and antiseptics to control the growth of bacteria such as Escherichia Coli . The rate of response of the various agents is different depending on the disinfectant. Thus, the level of efficiency and effectiveness of the agent is analyzed as a way of determining the potential of the agent to interrupt and affect the growth rate of the bacteria.
Hypothesis
The hypothesis of the experiment is supposition. The premise is as follows "if Clorox/Bleach is effective in the growth control of Escherichia Coli then it is possible to decrease the growth rate of the bacteria."
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Methodology
The following methodology is in use in experimenting. Two main steps are involved in the investigation. They include selecting the lab stock bacteria that will be used in testing the agent and deciding on the agent that needs to undergo testing. The bacteria selected in the experiment is Escherichia Coli and the test agents if Clorox/Bleach. The dilution in the research is serial. In the dilution a given amount of the agent is added to the respective plates consecutively, and the final dilution amount is determined.
The stock of bacteria that will be used in testing the agent
The following steps are involved in the selection
1. Creation of a turbid suspension of the stock culture.
2. Creation of a serial dilution for the cell counts.
3. Production of tubes, each with sterile water.
4. Transfer the micro pipettor to the suspension for testing the tube that has the sterile water.
5. Transfer the aliquot to all the dilution plates and incubate for 24 hours at a temperature of 37 degrees.
6. Conduct the colony.
In the second phase of selecting the agent, the same procedure is done again. The colony count is done at the end of the experiment.
Results
cfu/ml of the inoculum
To determine the inoculum's cfu/ml, a plate with more than thirty and less than three-hundred colonies is in identification. The average amount is the strategy for identifying the plate with colonies. Then the following formula will be used
Cfu/ml of the inoculum = (Number of settlements that are present in the plate/ the volume of the plate in ml) * (1/ the dilution plate).
The assumption in the computation is that 1ml was the amount that was on the plate. The plate selected is the one with a final dilution of 10 -5 . At 10 -4 there are too many colonies to count. At 10-6 the settlements are also few, and they are less than 30. The 10 -5 final dilution appears to be having the average number of colonies that can easily be in identification. The number of settlements on the plate is 230. Thus, the Cfu/ml of the inoculum will be 2.3*106.
Maximum dilution at which the agent was fully effective
From observing the plates, the maximum dilution in which the agent is fully active is Escherichia Coli and bleach 10 -3 . Such is because the dishes have no single colonies at that final dilution amount.
Discussion
The results resented in the peer-reviewed journal, and the outcomes of the investigation are comparable. That is because the findings of the two studies all have the same suggestion. In the peer-reviewed journal, the results indicate the effectiveness and efficiency of bleach on Escherichia Coli and the other three tested. The investigation conducted provides a similar suggestion as there is an absence of the Escherichia Coli colonies when bleach is in addition to the bacterium at a final dilution rate of 10 -3 . Thus. Both investigations suggest that bleach/Clorox is an effective and efficient agent towards Escherichia Coli bacteria. The survey's outcomes support the hypothesis stating that "if Clorox/Bleach is effective in the growth control of Escherichia Coli, then it is possible to decrease the growth rate of the bacteria." It is evident from the investigation that the agent has been effective in reducing the bacteria colonies. The reduction is from an average count of 2.3*106 to zero. The modification takes place after the addition and the dilution of the agent and bacteria. Most hospitals are already using bleach, such as dilute sodium hypochlorite in cleaning surfaces (Duong, 2017). Knowing that Clorox/Bleach is effective towards Escherichia Coli, the medical health care facilities reduce the bacteria from surfaces.
Conclusion
In conclusion, "if Clorox/Bleach is effective in the growth control of Escherichia Coli then it is possible to decrease the growth rate of the bacteria." A peer-reviewed journal suggests that bleach is good and efficient in harboring the growth of Escherichia Coli bacteria. The investigation is to determine is Clorox/bleach is effective towards Escherichia Coli. Serial dilution is in use in the experiment. In the dilution there is addition of 1ml of water to the bacteria culture to form the colonies. The colony count is where the range is between 30 and 300 colonies. In the investigation, the colony count was 2.3*106. The agent is in addition to the bacteria, and a plate in selection has no colonies. From the observation, the final dilution at which the dish had no settlements was 10 -3 . From the study's outcomes, it is clear that there are similarities with the peer-reviewed journal and the hypothesis of the investigation. Both studies suggest that bleach is an active agent towards the Escherichia Coli bacteria. From the experiment that is evident from the absence of colonies at the 10 -3 final dilution. Future studies should aim at making a comparison between the effectiveness of one bacterial agent to another. Such reviews will be useful in the determination of which agent is more effective than the other towards a specific bacterium.
References
Aldabbagh, S., Ali, H., Khalil, I. & Hamad, M. (2015). A Study of Some Antibiotics, Disinfectants and Antiseptics Efficacy Against some Species of Pathogenic Bacteria. Assiut Vet. Med. Journal, 61 (147), 210-217.
Duong, A. (2017). Effective Reduction of Bacteria from Surfaces Using Bleach and Silver Dihydrogen Citrate Solutions. Infection Control Tips. Retrieved from https://www.infectioncontrol.tips/2017/03/09/effective-reduction-bacteria-surfaces/
Han, J. H., Sullivan, N., Leas, B. F., Pegues, D. A., Kaczmarek, J. L., & Umscheid, C. A. (2015). Cleaning Hospital Room Surfaces to Prevent Health Care–Associated Infections: A Technical Brief. Annals of internal medicine, 163 (8), 598-607.
