The reason for the experiment is to ascertain and document unknown bacteria. The bacteria would be pinpointed following steps, tests and procedures learned in the microbiology laboratory class. Specific biochemical characteristics were used to identify and differentiate possible bacteria (Spratt, 2004). The importance of identifying unknown bacteria is to provide information with regard to the advantages and disadvantages of the bacteria in question. Bacteria are known for causing human diseases and infections while at the same time they are useful to human beings in that they are those found in food. The experiments help to provide the basis of research and innovations into possible future solutions.
There was the gram stain test that was used to establish which of the two bacteria would be gram positive and which one would be gram negative (Dash & Payyappilli, 2016). The DNase test was performed on the bacteria considered gram positive to test for the production of DNase which is usually negative for Staphylococci whereas the SIM test was excuted on the gram negative strain to test for sulfide production or indole formation. Afterwards, urea test was performed on the gram negative strain, whereas the SIM test was executed on the gram positive strain. The urea test was then carried out on the positive bacteria while the PRB lac test was conducted on the gram negative bacteria which tested both positive.
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Staphylococcus saproohyticus is a cocci shaped gram positive bacteria that is found in the female genitalia and the perineum (Kline & Lewis, 2016). The bacterium has been attributed to be the leading cause of Urinary Tract infections (Trivedie Science, 2015). Enterobacter aerogenes is a rod shaped gram negative bacteria that is found in the gastrointestinal tract of animals and in soil too (Grimont & Grimont, 2006). The bacterium is responsible for a majority of extra intestinal infections. These infections occur in areas such as respiratory system and the central nervous system.
References
Dash, C. & Payyappilli, D. (2016). KOH string and Vancomycin susceptibility test as an alternative method to Gram staining. Journal of International Medicine and Dentistry , 3 (2), 88-90.
Grimont, F. & Grimont, P. (2006). The Genus Enterobacter. Prokaryotes , 6, 197-214. Retrieved from: https://pdfs.semanticscholar.org/ac00/38d8d183dfaf7ca6f64b2551138e103f7180.pdf
Kline, K. A., & Lewis, A. L. (2016). Gram-Positive Uropathogens, Polymicrobial Urinary Tract Infection, and the Emerging Microbiota of the Urinary Tract. Microbiology Spectrum , 4(2), 10.1128/microbiolspec.UTI–0012–2012. http://doi.org/10.1128/microbiolspec.UTI-0012-2012
Spratt, David. (2004). Significance of Bacterial Identification by Molecular Biology Methods. Endodontic Topics , 9 (1), 5-14.
Trivedie Science. (2015). Antimicrobial Sensitivity, Biochemical Characteristics and Biotyping of Staphylococcus saprophyticus: An Impact of Biofield Energy Treatment. Women’s Healthcare. Retrieved from https://www.trivedieffect.com/the-science/publications/microbiology-publications/antimicrobial-sensitivity-biochemical-characteristics-and-biotyping-of-staphylococcus-saprophyticus-an-impact-of-biofield-energy-treatment/
